microencapsulation and fermentation of lactobacillus acidophilus la-5 and bifidobacterium bb-12

نویسندگان

maryam yari faculty of science, alzahra university, tehran, iran

jamshid fooladi faculty of science, alzahra university, tehran, iran

mohammad ali kargar motlagh iran dairy industrial co. (pegah-tehran), tehran, iran

چکیده

because of poor survival of probiotic bacteria, microencapsulation evolved from the immobilized cell culture technology used in the biotechnological industry. two probiotic strains, bifidobacterium (bb-12) and lactobacillus acidophilus (la-5) were immobilized in calcium alginate by extrusion method. encapsulation parameters and efficacy of this method were evaluated. growth factors of these two bacteria were also measured by culturing in 10-l fermenter. growth curves were obtained with respect to optical density and dry biomass weight. encapsulation yield was over than 60% in each experiment. scanning electron microscopy (sem) of entrapment of cells in alginate matrix and cross-sections of dried bead were obtained and illustrated . bifidobacterium have been shown better biotechnological properties.

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عنوان ژورنال:
applied food biotechnology

جلد ۲، شماره ۴، صفحات ۲۷-۳۲

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